YBCH-800 Auto Chemistry Analyzer
12/20/2019

Real Time PCR

As a necessary choice for quantitative analysis of molecular biology, real-time PCR system has been widely used in various fields such as scientific research, clinical detection and diagnosis, quality and safety testing, and forensic applications.

Feature

  • Up to 6 fluorescence detection channels allowing multiplex PCR.
  • Effectively reduce multi-color crosstalk and edge effect, no ROX correction required.
  • New optical scanning detection system
  • Innovative scanning method and time-resolved signal separation technology
  • Unique edge temperature compensation technology
  • User-friendly software

Technical Parameters

Temperature control system Detection system
Sample capacity 0.1ml PCR tubes×96, 8×12 PCR plate or 96 well plate x 1 Excitation light source 4/6 monochrome high efficiency LEDs
Reaction volume 10-50μl Detection device PMT
Thermal cycle technology Peltier Detection mode Time-resolved signal Time-resolved signal
Max. Heating/Cooling rate 6.0° C/s Excitation/detection wavelength RANGE 455-650nm/510-715nm
Heating temperature range 4 – 100 °C Fluorescent channels 4/6 channels
Temperature accuracy ± 0.2°C Supported dye FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ
Temperature uniformity ±0.2℃ @60℃ , ±0.2℃ @95℃ Sensitivity Single copy gene
Temperature gradient setting range 30–100°C Resolution 1.33 folds copy number difference can be distinguished in single-plex qPCR
Temperature gradient difference setting range 1 – 36°C Dynamic range 10 orders of magnitude copies